Summary
Abstract
Spray-application of membraneless acidic electrolyzed water (MLAEW) is a novel technique for disinfection in livestock houses. This study investigated the loss of free chlorine (FC – the major germicidal component in MLAEW) over distance during spraying, as affected by air temperature and initial FC concentration. The anti-microbial effect of MLAEW on airborne bacteria from an aviary laying-hen house was examined. MATERIALS AND METHODS: MLAEW was prepared at two FC concentrations: app. 15 and 60 mg L , and sprayed at three air temperatures (18, 25, 32 C). The original MLAEW solution and MLAEW aerosols collected at 0, 25, and 50 cm from the spray nozzle were analyzed for FC concentrations. Bacteria were immersed into these MLAEW samples and numerated for viable count after 0.5, 2 and 5-min treatments. RESULTS: MLAEW aerosols collected at 0 cm lost 11.7-13.2% FC, compared with the original MLAEW solution. This initial loss was affected neither by the initial FC concentration (P = 0.13) nor by air temperature (P = 0.57). The rate of FC loss during travelling was 0.79-0.87 % per cm of aerosol travel distance (% cm ) at 18 C, 1.08-1.15 % cm at 25 C, and 1.35-1.49% cm at 32 C. This travelling loss was affected by air temperature (P = 0.02), but not by initial FC concentration (P = 0.38). Bacteria were completely inactivated at 0.5 min when treated with MLAEW samples with FC > 16.8 mg L , in 2 min when FC > 13.8 mg L , and in 5 min when FC > 7.2 mg L . CONCLUSION: Airborne bacteria from aviary hen house can be effectively inactivated by MLAEW with adequate FC concentration and contact time. During spraying, the anti-microbial efficacy of MLAEW aerosols decreased over distance due to FC loss which exacerbated at higher air temperatures.
Study details
- Published in: Annals of Agricultural and Environmental Medicine 21.2 (2014)
- Microbes studied: Total Microbial Count
Source
Source: Annals of Agricultural and Environmental Medicine 21.2 (2014)
Conditions tested
| Parameter | Value |
|---|---|
| Organisms | Total microbial count |
| Relevant sectors | Livestock |
| Publication | Annals of Agricultural and Environmental Medicine, 2014 |
Reading this responsibly
Published figures are obtained under controlled conditions, usually on clean surfaces or in defined matrices. Organic load, surface condition, temperature and application coverage all affect real-world performance. Use these numbers to design a protocol, then validate it on your own site with environmental swabbing.
Applying it
Concentrations in this range are within the adjustable output of every EcoloxTech commercial system, so implementing this protocol is a settings question rather than a procurement one. Where the claim needs to sit on a registered label, see HOCl-AS200, EPA Reg. No. 101112-1.