Summary
Abstract
Electrolysis of a 0.1% (17.1 mM) solution of NaCl using separate anode and cathode compartments gives rise to solutions containing active chemical species. The strongly acidic anode solution (EW(+)) has high levels of dissolved oxygen and available chlorine in a form of hypochlorous acid (HOCl) with a strong potential for sterilization, which we have investigated here. Exposing Aspergillus parasiticus at an initial density of 103spores in 10 L to a 50-fold volume (500 L) of EW(+) containing ca. 390 mol HOCl for 15 min at room temperature resulted in a complete inhibition of fungal growth, whereas the cathode solution (EW( )) had negligible inhibitory effects. Moreover, the mutagenicity of aflatoxin B1 (AFB1) for Salmonella typhimurium TA-98 and TA-100 strains was strongly reduced after AFB1 exposure to the EW(+) but not with the EW( ). In high-performance liquid chromatography analysis, the peak corresponding to AFB1 disappeared after treatment with the EW(+), indicating decomposition of the aflatoxin. In contrast, the routinely used disinfectant sodium hypochlorite, NaOCl, of the same available chlorine content as that of EW(+) but in a different chemical form, hypochlorite (OCl-) ion, did not decompose AFB1 at pH 11. However, NaOCl did decompose AFB1 at pH 3, which indicated that the principle chemical formula to participate in the decomposition of AFB1 is not the OCl- ion but HOCl. Furthermore, because the decomposition of AFB1 was suppressed by pretreating the EW(+) with the OH radical scavenger thiourea, the chemical species responsible for the AFB1-decomposing property of the EW(+) should be at least due to the OH radical originated from HOCl. The OH in EW(+) was proved by electron spin resonance analysis.
Study details
- Published in: Journal of Agricultural and Food Chemistry 50.3 (2002): 633-641
- Microbes studied: Fungi, Aspergillus parasiticus, aflatoxin B1
Source
Source: Journal of Agricultural and Food Chemistry 50.3 (2002): 633-641
Conditions tested
| Parameter | Value |
|---|---|
| Organisms | Aspergillus spp., Fungi (general) |
| Relevant sectors | Agriculture & Livestock, Food Processing |
| Publication | Journal of Agricultural and Food Chemistry, 2002 |
Reading this responsibly
Published figures are obtained under controlled conditions, usually on clean surfaces or in defined matrices. Organic load, surface condition, temperature and application coverage all affect real-world performance. Use these numbers to design a protocol, then validate it on your own site with environmental swabbing.
Applying it
Concentrations in this range are within the adjustable output of every EcoloxTech commercial system, so implementing this protocol is a settings question rather than a procurement one. Where the claim needs to sit on a registered label, see HOCl-AS200, EPA Reg. No. 101112-1.