Summary
Abstract
Electrolyzed – oxidizing (EO) water is a relatively new method that has been utilized for killing pathogens in agriculture, medical sterilization and food sanitation. This water is generated by passing sodium chloride solution through EO water generator. In this study, the EO water was used to treat holy basil inoculated with Escherichia coli. The initial pH and oxidation – reduction potential (ORP) of EO water were 2.09 and 1200 mV, respectively. The treatments changed ORP to 800,950 and 1100 mV. The contact times were 10,30 and 60 min. In pure culture, E. coli viable counts in the sample treated with EO water were reduced to undetectable levels at all ORP and times. However no reduction in E. coli counts was achieved in the control sample (treated with deionized water). The initial population of E. coli was about 8.5 log10 CFU / ml which was inoculated on 5 g of holy basil. Results showed that the treatment treated with EO water was reduced about 2 log10 CFU / ml in ORP 800 and 950 mV, 4 log10 CFU / ml in ORP 1100 mV for 10 min. When the contact time increased to 30 min, the reduction of E. coli count was about 3 log 10 CFU / ml in ORP 950 mV and 5 log10 CFU / ml in ORP 1100 mV. But the reduction was not different from 10 min when treated with ORP 800 mV. When the contact time increased to 60 min, the reduction of E. coli count was about 3 log10 CFU / ml in ORP 800 mV, 4 log10 CFU / ml in ORP 950 mV. and 6 log10 CFU / ml in ORP 1100 mV. These results could be concluded that the ORP of EO water and contact time significantly inactivated E. coli.
Study details
- Published in: KASETSART JOURNAL (2006): 716
- Microbes studied: Escherichia coli
Source
Source: KASETSART JOURNAL (2006): 716
Conditions tested
| Parameter | Value |
|---|---|
| Organisms | Escherichia coli |
| Relevant sectors | Food Processing, Produce & Packing |
| Publication | KASETSART JOURNAL (2006): 716, 2006 |
Reading this responsibly
Published figures are obtained under controlled conditions, usually on clean surfaces or in defined matrices. Organic load, surface condition, temperature and application coverage all affect real-world performance. Use these numbers to design a protocol, then validate it on your own site with environmental swabbing.
Applying it
Concentrations in this range are within the adjustable output of every EcoloxTech commercial system, so implementing this protocol is a settings question rather than a procurement one. Where the claim needs to sit on a registered label, see HOCl-AS200, EPA Reg. No. 101112-1.